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Items: 3
1.
Whitworth KM, Rowland RRR, Petrovan V, Sheahan M, Cino-Ozuna AG, Fang Y, Hesse R, Mileham A, Samuel MS, Wells KD, Prather RS.
Transgenic Res. 2019 Feb;28(1):21-32. doi: 10.1007/s11248-018-0100-3. Epub 2018 Oct 12.
- PMID:
- 30315482
2.
Vijgen L, Keyaerts E, Zlateva K, Van Ranst M.
Int J Infect Dis. 2004 Jul;8(4):217-22.
Int J Infect Dis. 2004 Jul;8(4):217-22.
Identification of six new polymorphisms in the human coronavirus 229E receptor gene (aminopeptidase N/CD13).Abstract
OBJECTIVE:
Human aminopeptidase N (APN/CD13/ANPEP) has been identified as the receptor for human coronavirus (HCoV) 229E. In this study, we analyzed the region of the APN gene that encodes a stretch of amino acid residues, essential for its HCoV-229E receptor function (amino acids 260-353).
METHODS:
Full-length APN exon 3, intron 3 and exon 4, was PCR-amplified and sequenced in DNA samples from 100 unrelated Caucasian Belgian healthy volunteers.
RESULTS:
We identified seven polymorphisms, including four intron 3 and three exon 4 variations. Apart from the already known C956T exon 4 mutation, the six other polymorphisms have not yet been described. The most prevalent APN variations in this population (C956T leading to an alanine to valine substitution, G978T, G987A and intron3-C389T) always occurred together at an allele frequency of 8.5%. Haploid DNA sequencing demonstrated the presence of these four variations on the same allele. Three polymorphisms in intron 3, intron3-G395C, intron3-C86T, and intron3-C429T, were identified with an allele frequency of 3.5%, 1% and 0.5% respectively. Five haplotypes were identified in the population of 100 individuals.
Free Article
3.
Luo Y, Jiang L, Ao X, Lu Z, Liu HD, Xu Y, Ao Y, Ren Q, Lu C, Xu HM, Zhang X.
Yi Chuan Xue Bao. 2003 Jul;30(7):687-92. Chinese.
Yi Chuan Xue Bao. 2003 Jul;30(7):687-92.
[Genomic variations in the locus for aminopeptidase N:a putative cellular receptor for SARS-CoV spike glycoprotein].
[Article in Chinese]
Abstract
Aminopeptidase N has been identified as the cellular receptor for human coronavirus HCoV-229E and was a putative receptor for the spike glycoprotein encoded by the SARS-associated coronavirus (SARS-CoV). We report here identification of 9 single nucleotide polymorphisms (SNPs) in ANPEP, encoding human aminopeptidase N, in Chinese. All ANPEP
exons and their flanking intronic sequences were amplified from
unrelated normal individuals by polymerase chain reaction (PCR) and
screened using denaturing high-performance liquid chromatography
(DHPLC). Nine SNPs were revealed after direct sequencing of PCR
amplified fragments which showed changes of DHPLC chromatogram. Four of
these polymorphisms, T321M(962C > T), S651L(1952C > T),
S752N(2255G > A) and G764R(2290G > A), were non-synonymous; the
remaining exonic synonymous and intronic ones were T795T(2385C > T),
IVS7 + 17G > A, IVS14-16A > G, IVS17 + 12C > G and IVS17 + 44C
> T. Our data may be useful for studies to investigate the role of
host genetic factors in SARS pathogenesis, especially for identifying
SARS-susceptible and/or anti-SARS alleles.
- PMID:
- 14579541
- [Indexed for MEDLINE]
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