Hermonvälittäjäaineitten simultaani analyysi

 Samanaikaisanalyysi kahdestakymmnestä hermonvälittäjäaineesta. Merkkaan luettelossa mustalla koroostettuina ne jotka johtuvat aminohappofenylalaniinsita( ja tyrosiinista ) käsin. Tyrosiinin suora dekarboksylaatiotuote (  aminohappo miinus hiilidioksidi)  on tyramiini, jolla varsinaisesti ei ole funktiota, muyta sitä syntyy tässä  aineenvaihdunnassa ja se esiintyy  jalostettujen hermonvälittäjäaineityen joukossa ja saaytaa tehostaa niitten signalointia. Se liikkuu passiivilla diffuusiolla ilman omaa kuljettajaa tai reseptoria.

http://www.ncbi.nlm.nih.gov/pubmed/25869798

J Chromatogr A. 2015 May 22;1395:79-87. doi: 10.1016/j.chroma.2015.03.056. Epub 2015 Mar 30. Simultaneous analysis of multiple neurotransmitters by hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry.

Tufi S¹, Lamoree M², de Boer J², Leonards P².

Tiivistelmä , Abstract

Neurotransmitters are endogenous metabolites that allow the signal transmission across neuronal synapses. Their biological role is crucial for many physiological functions and their levels can be changed by several diseases. Because of their high polarity, hydrophilic interaction liquid chromatography (HILIC) is a promising tool for neurotransmitter analysis. Due to the large number of HILIC stationary phases available, an evaluation of the column performances and retention behaviors has been performed on five different commercial HILIC packing materials (silica, amino, amide and two zwitterionic stationary phases). Several parameters like the linear correlation between retention and the distribution coefficient (logD), the separation factor k and the column resolution Rs have been investigated and the column performances have been visualized with a heat map and hierarchical clustering analysis. An optimized and validated HILIC-MS/MS method based on the ZIC-cHILIC column is proposed for the simultaneous detection and quantification of twenty compounds consisting of neurotransmitters, precursors and metabolites:

1.  3-methoxytyramine (3-MT), 
2. 5-hydroxyindoleacetic acid (5-HIAA),
3. 5-hydroxy-L-trypthophan, 
4. acetylcholine,
5. choline,
6.  L-3,4-dihydroxyphenylalanine (L-DOPA),
7.  dopamine,
8.  epinephrine,
9.  γ-aminobutyric acid (GABA),
10.  glutamate,
11.  glutamine,
12.  histamine,
13.  histidine,
14.  L-tryptophan,
15.  L-tyrosine,
16.  norepinephrine,
17.  normetanephrine,
18.  phenylalanine,
19.  serotonin
20.  tyramine.

 The method was applied to neuronal metabolite profiling of the central nervous system of the freshwater snail Lymnaea stagnalis. This method is suitable to explore neuronal metabolism and its alteration in different biological matrices.
Copyright © 2015 Elsevier B.V. All rights reserved.