https://journals.asm.org/doi/10.1128/jvi.77.5.3334-3338.2003
Development of VP30-targeted nanoparticles using DPS4 fusion peptides for the inhibition of Ebola virus.
J Nanobiotechnology. 2026 Mar 11;24(1):371. doi: 10.1186/s12951-026-04260-1.PMID: 41814375 Free PMC article.
VP30, a critical RNA synthesis factor, interacts with nucleoprotein (NP) to facilitate Ebola viral genome transcription and replication. ...Antiviral effects were evaluated using minigenome and transcription and replication-competent virus-like particl …
The Ebolavirus Transcription Regulatory Signal Equilibrates Between Two Structural Conformations to Affect VP30 Binding.
J Mol Biol. 2025 Dec 15;437(24):169464. doi: 10.1016/j.jmb.2025.169464. Epub 2025 Sep 25.PMID: 41015113
The transcription of ebolavirus (EBOV) genomes is a major checkpoint in the viral life cycle. ...Mutational studies demonstrate that the entire hairpin structure is important in directing both VP30 binding and regulating EBOV transcriptional activit …
Computational study of interaction of calixarene with ebola virus structural proteins and its potential therapeutic implications.
J Mol Graph Model. 2025 May;136:108976. doi: 10.1016/j.jmgm.2025.108976. Epub 2025 Feb 12.PMID: 39961277
Ebola virus (EBOV) is a negative-strand RNA virus that causes hemorrhagic fever and fatal illness in humans. According to WHO, the Ebola virus caused 28,646 fatal cases and 11,323 deaths in West Africa due to hemorrhagic fever and deadly …
To be or not to be phosphorylated: understanding the role of Ebola virus nucleoprotein in the dynamic interplay with the transcriptional activator VP30 and the host phosphatase PP2A-B56.
Emerg Microbes Infect. 2025 Dec;14(1):2447612. doi: 10.1080/22221751.2024.2447612. Epub 2025 Jan 12.PMID: 39726359 Free PMC article.
Ebola virus (EBOV) transcription is essentially regulated via dynamic dephosphorylation of its viral transcription activator VP30 by the host phosphatase PP2A. ...This study investigates NP's role in VP30 dephosphorylation and transcri …
Ginkgolic acid inhibits Ebola virus transcription and replication by disrupting the interaction between nucleoprotein and VP30 protein.
Antiviral Res. 2025 Feb;234:106074. doi: 10.1016/j.antiviral.2024.106074. Epub 2024 Dec 22.PMID: 39716669
The antiviral efficacy of ginkgolic acid was demonstrated in the EBOV transcription- and replication-competent virus-like particle (trVLP) system. Furthermore, we resolved the crystal structure of the VP30-ginkgolic acid complex, revealing two ginkgolic acid molecul …https://www.genecards.org/cgi-bin/carddisp.pl?gene=RBBP6&keywords=RBBP6
The cellular protein phosphatase 2A is a crucial host factor for Marburg virus transcription.
J Virol. 2024 Sep 17;98(9):e0104724. doi: 10.1128/jvi.01047-24. Epub 2024 Aug 28.PMID: 39194238 Free PMC article.
Previous studies assumed that dephosphorylation of the filoviral transcription factor VP30 supports transcription, while phosphorylated VP30 reduces transcription. Here, we focused on the role of the host protein phosphatase 2A (PP2A) for VP3 …
Hippo signaling pathway regulates Ebola virus transcription and egress.
Nat Commun. 2024 Aug 13;15(1):6953. doi: 10.1038/s41467-024-51356-z.PMID: 39138205 Free PMC article.
Here, we identify LATS1/2 kinases and YAP, key components of the Hippo pathway, as critical regulators of EBOV transcription and egress. Specifically, we find that when YAP is phosphorylated by LATS1/2, it localizes to the cytoplasm (Hippo "ON") where it sequesters VP40 to …
Development of a Měnglà virus minigenome and comparison of its polymerase complexes with those of other filoviruses.
Virol Sin. 2024 Jun;39(3):459-468. doi: 10.1016/j.virs.2024.03.011. Epub 2024 May 21.PMID: 38782261 Free PMC article.
Ebola virus (EBOV) and Marburg virus (MARV), members of the Filoviridae family, are highly pathogenic and can cause hemorrhagic fevers, significantly impacting human society. ...In this study, we used RACE technology to sequence the 3'-leader and 5'-trailer of Mengla virus…https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04da/11279764/b959835795f8/gr1.gif
ICTV Virus Taxonomy Profile: Filoviridae 2024.
J Gen Virol. 2024 Feb;105(2):001955. doi: 10.1099/jgv.0.001955.PMID: 38305775 Free PMC article.
The filovirid genome is a linear, non-segmented RNA with five canonical open reading frames (ORFs) that encode a nucleoprotein (NP), a polymerase cofactor (VP35), a glycoprotein (GP(1,2)), a transcriptional activator (VP30) and a large protein (L) containing …
N(6)-methyladenosine is required for efficient RNA synthesis of Ebola virus and other haemorrhagic fever viruses.
PMID: 37306620 Free PMC article.N6-methyladenosine (m6A) is one of the most abundant modifications of cellular RNA, where it serves various functions. m6A methylation of many viral RNA species has also been described; however, little is known about the m6A epitranscriptome of haemorrhagic fever-causing viruses like Ebola virus (EBOV). Here, we analysed the importance of the methyltransferase METTL3 for the life cycle of this virus. We found that METTL3 interacts with the EBOV nucleoprotein and the transcriptional activator VP30 to support viral RNA synthesis, and that METTL3 is recruited into EBOV inclusions bodies, where viral RNA synthesis occurs. Analysis of the m6A methylation pattern of EBOV mRNAs showed that they are methylated by METTL3. Further studies revealed that METTL3 interaction with the viral nucleoprotein, as well as its importance for RNA synthesis and protein expression, is also observed for other haemorrhagic fever viruses such as Junín virus (JUNV) and Crimean-Congo haemorrhagic fever virus (CCHFV). The negative effects on viral RNA synthesis due to loss of m6A methylation are independent of innate immune sensing, as METTL3 knockout did not affect type I interferon induction in response to viral RNA synthesis or infection. Our results suggest a novel function for m6A that is conserved among diverse haemorrhagic fever-causing viruses (i.e. EBOV, JUNV and CCHFV), making METTL3 a promising target for broadly-acting antivirals.
Keywords: Crimean-Congo haemorrhagic fever virus; Ebola virus; Junín virus; METTL3; arenavirus; filovirus; m6A; orthonairovirus.
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