IFI16,

 https://www.genecards.org/cgi-bin/carddisp.pl?gene=IFI16

• GeneCards Symbol: IFI16 2 
• Interferon Gamma Inducible Protein 16 2 3 5
• IFNGIP1 2 3 4 5
• PYHIN2 2 3 5
• Interferon-Inducible Myeloid Differentiation Transcriptional Activator 3 4
• Gamma-Interferon-Inducible Protein 16 3 4
• Interferon-Gamma Induced Protein IFI 16 3
• Ifi-16

• Post-translational modifications for IFI16 Gene

  • Ubiquitinated by human herpes simplex virus 1 (HHV-1) ICP0 protein; leading to degradation by the proteasome. ( Q16666-IF16_HUMAN )
  • Lysine acetylation in the multipartite nuclear localization signal (NLS) regulates the subcellular location.
    In vitro can be acetylated by p300/EP300 coupled to cytoplasmic localization.
    ( Q16666-IF16_HUMAN )
  • Phosphorylated on Ser and Thr. ( Q16666-IF16_HUMAN )
  • Isoform 3 seems to show a minor degree of complex carbohydrate addition. ( Q16666-IF16_HUMAN )
  • Glycosylation at Asn178 ( NX_Q16666 [NX_Q16666-1] )
  • Ubiquitination at Lys128, Lys190, Lys143, Lys614, and Lys743 ( NX_Q16666 [NX_Q16666-1] )
  • Modification sites at PhosphoSitePlus ( Q16666 )
  • Glycosylation from GlyConnect
    • IF16_HUMAN (1263)
  • Glycosylation from GlyGen (Q16666) 4 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (2 sites)

• Gene Families for IFI16 Gene

  HGNC:

  • Pyrin and HIN domain family
  • Pyrin domain containing

  IUPHAR:

  • Absent in melanoma (AIM)-like receptors (ALRs)

  The Human Protein Atlas (HPA):

  • Plasma proteins
  • Predicted intracellular proteins

  Protein Domains for IFI16 Gene

  InterPro:

  • DAPIN 
   
  • DEATH-like_dom_sf 
   
  • HIN-200 
   
  • HIN200/IF120x 
   
  • NA-bd_OB-fold

  Blocks:

  • Pyrin domain 
   
  • HIN-200/IF120x domain

  Suggested Antigen Peptide Sequences for IFI16 Gene

  GenScript: Design optimal peptide antigens:

  • Interferon, gamma-inducible protein 16, isoform CRA_b (D3DUZ3_HUMAN)
  • Interferon-inducible myeloid differentiation transcriptional activator (IF16_HUMAN)

  Graphical View of Domain Structure for InterPro Entry

  Q16666

  UniProtKB/Swiss-Prot:

  IF16_HUMAN :

  • The HIN-200 domains mediates dsDNA binding via electrostatic interactions.
  • Belongs to the HIN-200 family.

  Domain:

  • The HIN-200 domains mediates dsDNA binding via electrostatic interactions.

  Family:

  • Belongs to the HIN-200 family.

  Molecular function for IFI16 Gene according to UniProtKB/Swiss-Prot

  Function:

  • Binds double-stranded DNA.
    Binds preferentially to supercoiled DNA and cruciform DNA structures.
    Seems to be involved in transcriptional regulation.
    May function as a transcriptional repressor.
    Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes.
    Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein.
    May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status.
    Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy.
    May be involved in regulation of TP53-mediated cell death also involving BRCA1.
    May be involved in the senescence of prostate epithelial cells.
    Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus.
    After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta.
    Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2.
    Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD.
    Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors.
    Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication.
    Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. IF16_HUMAN,Q16666
  • [Isoform IFI16-beta]: Isoform that specifically inhibits the AIM2 inflammasome (PubMed:30104205).
    Binds double-stranded DNA (dsDNA) in the cytoplasm, impeding its detection by AIM2 (PubMed:30104205).
    Also prevents the interaction between AIM2 and PYCARD/ASC via its interaction with AIM2, thereby inhibiting assembly of the AIM2 inflammasome (PubMed:30104205).
    This isoform also weakly induce production of type I interferon-beta (IFNB1) via its interaction with STING1 (PubMed:30104205). IF16_HUMAN,Q16666

  Induction:

  • Strongly induced by IFNG/IFN-gamma and, to a lesser extent, by alpha interferon.
    In HL-60 cells, maximum induction by IFNG/IFN-gamma occurs within 12 hours whereas, for IFN-alpha, only 10-fold induction was observed after 36 hours.
    Induced in vitro by dimethylsulfoxide, retinoic acid and 1,25 dihydroxyvitamin D3.
    Induced in monocytes by IFN-alpha and viral dsDNA.
    Induced by glucose restriction. IF16_HUMAN,Q16666
  • [Isoform IFI16-beta]: By interferon-beta (IFNB1). IF16_HUMAN,Q16666

  Molecular function for IFI16 Gene according to GENATLAS

  Biochemistry:

  • nuclear protein,constitutively expressed in lymphocytes,interferon alpha or gamma inducible in myeloid cells (clone IFI-6-16) IFI16