ZIC1 on pikkuaivojen sinkkisormiproteiini

 https://www.sciencedirect.com/science/article/abs/pii/S0147027220300349

• Pikkuaivojen sinkkisormiproteiinin ZIC1  geenitieto: Artikkeli mainitsee  GLI-kaltaisia C2H2 tyyppisiä  sinkkiproteiineja.  Etsin  niiden geenitietoja.

 https://www.genecards.org/cgi-bin/carddisp.pl?gene=ZIC1&keywords=ZIC1

ZIC sinkkisormiproteiinejakin on ainakin  5 geeniä.  

•  ARTIKKELI, jossa näitä ZIC ja GLI sinkkisormiproteiineja mainitaan usea. Niiden  sinkkisormityyppi on C2H2.

Roles and correlation of FOXA1 and ZIC1 in breast cancer

Author links open overlay panel et aliae.

The aim of this study was to evaluate the prognostic role of Forkhead box A1 (FOXA1) in breast cancer and determine the relationship between FOXA1 and zinc finger of the cerebellum 1 (ZIC1). 

 

BCIP, GEPIA, and Oncomine databases were used to detect expression of FOXA1 and assess prognostic roles of FOXA1 and ZIC1 in invasive breast tumors. A total of 113 female invasive breast cancer cases were collected to investigate FOXA1 and ZIC1 expression via immunohistochemistry. Twenty pairs of frozen-thawed tumors were used to select reliable indicators via western blotting and real-time quantitative polymerase chain reaction. In addition, Kaplan-Meier curves and Cox regression analysis were performed to analyze the overall survival (OS) and relapse-free survival.

 

 Multiple databases showed that FOXA1 expression was elevated in invasive breast cancer and negatively related to ZIC1. 

BCIP database also displayed a poor prognosis of high FOXA1 and low ZIC1. FOXA1 was positively associated with tumor size, grading, lymph node metastasis, and Tumor Node Metastasis (TNM) staging, while ZIC1 expression was negatively related to grading, lymph node metastasis, and TNM staging. 

 https://www.genecards.org/cgi-bin/carddisp.pl?gene=FOXA1&keywords=FOXA1

In Kaplan-Meier and Cox regression analysis, FOXA1 negative group and ZIC1 positive group had better OS rate and recurrence-free survival rate. In addition, a joint evaluation showed that “FOXA1- ZIC1+” had the highest OS and relapse-free survival, but “FOXA1+ ZIC1-” had the lowest ones. FOXA1 was negatively related to ZIC1 in breast cancer and they had different roles in clinicopathology and prognosis. 

 

Combined examination of FOXA1 and ZIC1 could bring more benefit to breast cancer patients.

Introduction

As one of the most common malignancies in women, breast cancer (BC) is severely threatening the human health and social development.¹ However, rapid progress, recurrence, and drug resistance are still the obstacles in the process of anticancer treatment, in the result of the pathogenesis of BC remains obscure.² Thus, further researches should be conducted to explore mechanisms of BC carcinogenesis in order to improve the prognosis of BC patients.

 

Forkhead box A1 (FOXA1) belongs to the forkhead box family of transcription factors, containing a forkhead DNA-binding domain.³ FOXA1 is not only essential for development and functional integrity of human various tissues, but also crucial for the development of carcinomas.4, 5, 6, 7 Recent studies have shown that FOXA1 can become a potential oncogene in the development of tumors, like hepatocellular carcinoma, non–small cell lung cancer and glioma.8, 9, 10

 

 In BC, researchers demonstrated that FOXA1 was a promising candidate as a therapeutic and prognostic target.11, 12 However, they disagreed with the prognostic role of FOXA1.13, 14

Zinc finger of the cerebellum 1 (ZIC1), a member of ZIC family, is essential for development of human nervous system, in the result of its 5 Cys2His2 zinc-finger domains interacting with the Gli family proteins.¹⁵ Recently, reports have argued that ZIC1 is putative tumor-suppressor gene in various neoplasms, including BC.^16,17 Its downregulation has been associated with worse prognosis in patients with BC.¹⁸

 GLI1 , zinc finger protein ,  https://www.genecards.org/cgi-bin/carddisp.pl?gene=GLI1&keywords=GLI1

GLI2, zinc finger protein , https://www.genecards.org/Search/Keyword?queryString=GLI2

GLI3 https://www.genecards.org/cgi-bin/carddisp.pl?gene=GLI3&keywords=GLI3

In public databases, a significantly negative relationship between FOXA1 and ZIC1 can be found (Supplementary 1). This indicates that overexpressed FOXA1 may be related to development of BC. One study also found a higher expression of FOXA1 in BC compared with matched adjacent normal breast tissues.¹⁹ Therefore, we designed this study to assess the prognostic role of FOXA1 in BC and determine the relationship between FOXA1 and ZIC1.